免疫系统相关疾病

胚胎干细胞打靶

1Mombaerts P; Iacomini J; Johnson RS; Herrup K; Tonegawa S; Papaioannou VE. 1992. RAG-1-deficient mice have no mature B and T lymphocytes. Cell 68(5):869-77. 2Alexander CE; Kaye PM; Engwerda CR. 2001. CD95 is required for the early control of parasite burden in the liver of Leishmania donovani-infected mice. Eur J Immunol 31(4):1199-210. 3Allen CD; Ansel KM; Low C; Lesley R; Tamamura H; Fujii N; Cyster JG. 2004. Germinal center dark and light zone organization is mediated by CXCR4 and CXCR5. Nat Immunol 5(9):943-52. 4Alugupalli KR; Leong JM; Woodland RT; Muramatsu M; Honjo T; Gerstein RM. 2004. B1b lymphocytes confer T cell-independent long-lasting immunity. Immunity 21(3):379-90.

传染病

转基因小鼠  

[1].Murakami T, Carmona N, Ono A. Virion-incorporated PSGL-1 and CD43 inhibit both cell-free infection and transinfection of HIV-1 by preventing virus-cell binding. Proc Natl Acad Sci U S A. 2020 Apr 7;117(14):8055-8063. doi: 10.1073/pnas.1916055117. Epub 2020 Mar 19. PMID: 32193343; PMCID: PMC7148576. [2].Dole VS, Bergmeier W, Patten IS, Hirahashi J, Mayadas TN, Wagner DD. PSGL-1 regulates platelet P-selectin-mediated endothelial activation and shedding of P-selectin from activated platelets. Thromb Haemost. 2007 Oct;98(4):806-12. PMID: 17938805.

传染病模型

靶基因PSGL-1&SCARB2均位于pXL-5载体的CMV启动子下游，由CMV启动子启动表达。

[1].Zhou D, Zhao Y, Kotecha A, Fry EE, Kelly JT, Wang X, Rao Z, Rowlands DJ, Ren J, Stuart DI. Unexpected mode of engagement between enterovirus 71 and its receptor SCARB2. Nat Microbiol. 2019 Mar;4(3):414-419. doi: 10.1038/s41564-018-0319-z. Epub 2018 Dec 10. PMID: 30531980. [2].Zhang X, Yang P, Wang N, Zhang J, Li J, Guo H, Yin X, Rao Z, Wang X, Zhang L. The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection. Protein Cell. 2017 Aug;8(8):590-600. doi: 10.1007/s13238-017-0405-7. Epub 2017 Apr 26. PMID: 28447294; PMCID: PMC5546930. [3].Murakami T, Carmona N, Ono A. Virion-incorporated PSGL-1 and CD43 inhibit both cell-free infection and transinfection of HIV-1 by preventing virus-cell binding. Proc Natl Acad Sci U S A. 2020 Apr 7;117(14):8055-8063. doi: 10.1073/pnas.1916055117. Epub 2020 Mar 19. PMID: 32193343; PMCID: PMC7148576. [4].Dole VS, Bergmeier W, Patten IS, Hirahashi J, Mayadas TN, Wagner DD. PSGL-1 regulates platelet P-selectin-mediated endothelial activation and shedding of P-selectin from activated platelets. Thromb Haemost. 2007 Oct;98(4):806-12. PMID: 17938805.

免疫系统相关疾病

胚胎干细胞打靶

1Colgan J; Asmal M; Neagu M; Yu B; Schneidkraut J; Lee Y; Sokolskaja E; Andreotti A; Luban J.. Cyclophilin A regulates TCR signal strength in CD4+ T cells via a proline-directed conformational switch in Itk. Immunity，2004，21(2):189-201. 2Bell RD; Winkler EA; Singh I; Sagare AP; Deane R; Wu Z; Holtzman DM; Betsholtz C; Armulik A; Sallstrom J; Berk BC; Zlokovic BV.. Apolipoprotein E controls cerebrovascular integrity via cyclophilin A. Nature，2012，485(7399):512-6. 3Colgan J; Asmal M; Luban J.. Isolation, characterization and targeted disruption of mouse ppia: cyclophilin A is not essential for mammalian cell viability Genomics，2000 ，68(2):167-78. Colgan J; Asmal M; Yu B; Luban J.. Cyclophilin A-deficient mice are resistant to immunosuppression by cyclosporine. J Immunol，2005，174(10):6030-8.

传染病小鼠模型

本项目为了建立HCV感染的小鼠模型，克隆了人的OCLN编码基因，将其插入pUBC载体的泛素基因启动子下游。

[1].Liu Z, He JJ. Cell-cell contact-mediated hepatitis C virus (HCV) transfer, productive infection, and replication and their requirement for HCV receptors. J Virol. 2013 Aug;87(15):8545-58. doi: 10.1128/JVI.01062-13. Epub 2013 May 29. PMID: 23720720; PMCID: PMC3719812. [2].Fénéant L, Ghosn J, Fouquet B, Helle F, Belouzard S, Vausselin T, Séron K, Delfraissy JF, Dubuisson J, Misrahi M, Cocquerel L. Claudin-6 and Occludin Natural Variants Found in a Patient Highly Exposed but Not Infected with Hepatitis C Virus (HCV) Do Not Confer HCV Resistance In Vitro. PLoS One. 2015 Nov 12;10(11):e0142539. doi: 10.1371/journal.pone.0142539. PMID: 26561856; PMCID: PMC4643007.

免疫系统相关疾病

干细胞打靶，破坏第3外显子

[1] Baidong Hou,Boris Reizis,and Anthony L. DeFranco1.Toll-like receptor-mediated dendritic cell-dependent and -independent stimulation of innate and adaptive immunity.Immunity. 2008 August 15; 29(2): 272–282.

免疫系统相关疾病

胚胎干细胞打靶

[1] McGehee AM, Strijbis K, Guillen E, Eng T, Kirak O, Ploegh HL. Ubiquitin-dependent control of class II MHC localization is dispensable for antigen presentation and antibody production. PLoS One. 2011 Apr 20;6(4):e18817. doi: 10.1371/journal.pone.0018817. PMID: 21533087; PMCID: PMC3080378.

免疫系统相关疾病

胚胎干细胞打靶，破坏第1和2外显子

[1].Russell HI, York IA, Rock KL, Monaco JJ. Class II antigen processing defects in two H2d mouse cell lines are caused by point mutations in the H2-DMa gene. Eur J Immunol. 1999 Mar;29(3):905-11.doi:10.1002/(SICI)1521-4141(199903)29:03<905::AID-IMMU905>3.0.CO;2-8. PMID: 10092094. [2].Chen X, Jensen PE. MHC class II antigen presentation and immunological abnormalities due to deficiency of MHC class II and its associated genes. Exp Mol Pathol. 2008 Aug;85(1):40-4. doi: 10.1016/j.yexmp.2008.03.011. Epub 2008 Apr 13. PMID: 18547561; PMCID: PMC2568888.

免疫系统相关疾病

胚胎干细胞打靶

[1] Honda K, Yanai H, Negishi H, Asagiri M, Sato M, Mizutani T, Shimada N, Ohba Y, Takaoka A, Yoshida N, Taniguchi T.IRF-7 is the master regulator of type-I interferon-dependent immune responses.Nature. 2005 Apr 7;434(7034):772-7. Epub 2005 Mar 30.

免疫系统相关疾病

自发突变品系 

1Andrews BS; Eisenberg RA; Theofilopoulos AN; Izui S; Wilson CB; McConahey PJ; Murphy ED; Roths JB; Dixon FJ.. Spontaneous murine lupus-like syndromes. Clinical and immunopathological manifestations in several strains. J Exp Med，1978，148(5):1198-215. 2Clark LD; Clark RK; Heber-Katz E. A new murine model for mammalian wound repair and regeneration. Clin Immunol Immunopathol，1998，88(1):35-45. 3Drappa J; Brot N; Elkon KB. The Fas protein is expressed at high levels on CD4+CD8+ thymocytes and activated mature lymphocytes in normal mice but not in the lupus-prone strain, MRL lpr/lpr. Proc Natl Acad Sci U S A，1993，90(21):10340-4. 4Fields ML; Sokol CL; Eaton-Bassiri A; Seo Sj; Madaio MP; Erikson J. Fas/fas ligand deficiency results in altered localization of anti-double-stranded dna b cells and dendritic cells. J Immunol，2001，167(4):2370-8. 5Gu L; Weinreb A; Wang XP; Zack DJ; Qiao JH; Weisbart R; Lusis AJ. Genetic determinants of autoimmune disease and coronary vasculitis in the MRL-lpr/lpr mouse model of systemic lupus erythematosus. J Immunol，1998，161(12):6999-7006. 6Hewicker M; Kromschroder E; Trautwein G. Detection of circulating immune complexes in MRL mice with different forms of glomerulonephritis. Z Versuchstierkd，1990，33(4):149-56. 7Johnson BC; Morton JI; Trune DR. Lacrimal and salivary gland inflammation in the C3H/Ipr autoimmune strain mouse: a potential mode for Sjogren's syndrome. Otolaryngol Head Neck Surg，1992，106(4):394-9. 8Kawase E; Suemori H; Takahashi N; Okazaki K; Hashimoto K; Nakatsuji N. Strain difference in establishment of mouse embryonic stem (ES) cell lines. Int J Dev Biol，1994， 38(2):385-90.

信号转导相关疾病

胚胎干细胞打靶

[1]Nguyen AQ, Sutley S, Koeppen J, Mina K, Woodruff S, Hanna S, Vengala A, Hickmott PW, Obenaus A, Ethell IM. Astrocytic Ephrin-B1 Controls Excitatory-Inhibitory Balance in Developing Hippocampus. J Neurosci. 2020 Sep 2;40(36):6854-6871. doi: 10.1523/JNEUROSCI.0413-20.2020. Epub 2020 Aug 12. PMID: 32801156; PMCID: PMC7470912. [2]Zhang H, Cui Z, Cheng D, Du Y, Guo X, Gao R, Chen J, Sun W, He R, Ma X, Peng Q, Martin BN, Yan W, Rong Y, Wang C. RNF186 regulates EFNB1 (ephrin B1)-EPHB2-induced autophagy in the colonic epithelial cells for the maintenance of intestinal homeostasis. Autophagy. 2021 Oct;17(10):3030-3047. doi: 10.1080/15548627.2020.1851496. Epub 2020 Dec 17. PMID: 33280498. [3]Nguyen AQ, Koeppen J, Woodruff S, Mina K, Figueroa Z, Ethell IM. Astrocytic Ephrin-B1 Controls Synapse Formation in the Hippocampus During Learning and Memory. Front Synaptic Neurosci. 2020 Mar 17;12:10. doi: 10.3389/fnsyn.2020.00010. PMID: 32256333; PMCID: PMC7092624.

免疫系统相关疾病

ENU诱变

1Randall KL, Lambe T, Johnson A, Treanor B, Kucharska E, Domaschenz H, Whittle B, Tze LE, Enders A, Crockford TL, Bouriez-Jones T, Alston D, Cyster JG, Lenardo MJ, Mackay F, Deenick EK, Tangye SG, Chan TD, Camidge T, Brink R, Vinuesa CG, Batista FD, Cornall RJ, Goodnow CC. Dock8 mutations cripple B cell immunological synapses, germinal centers and long-lived antibody production. Nat Immunol，2009，10(12):1283-91. 2Griggs, B. L., Ladd, S., Saul, R. A., DuPont, B. R., Srivastava, A. K. Dedicator of cytokinesis 8 is disrupted in two patients with mental retardation and developmental disabilities. Genomics, 2008，91: 195-202. 3MacDermot, K. D., Hulten, M. Female with hypohidrotic ectodermal dysplasia and de novo (X;9) translocation: clinical documentation of the AnLy cell line case. Hum. Genet, 1990，84: 577-579. 4Ruusala, A., Aspenstrom, P. Isolation and characterisation of DOCK8, a member of the DOCK180-related regulators of cell morphology. FEBS Lett, 2004，572: 159-166. 5Zhang, Q., Davis, J. C., Lamborn, I. T., Freeman, A. F., Jing, H., Favreau, A. J., Matthews, H. F., Davis, J., Turner, M. L., Uzel, G., Holland, S. M., Su, H. C. Combined immunodeficiency associated with DOCK8 mutations. New Eng. J. Med., 2009，361: 2046-2055.