B6.Cg-Tg(TcraTcrb)425-

从包含内源性TCR启动子的T细胞杂交瘤克隆中提取含有T细胞受体α链和β链的转基因结构被共注射到受精卵中。

1Barnden MJ; Allison J; Heath WR; Carbone FR.. Defective TCR expression in transgenic mice constructed using cDNA-based alpha- and beta-chain genes under the control of heterologous regulatory elements. Immunol Cell Biol，1998，76(1):34-40. 2Parameswaran N; Samuvel DJ; Kumar R; Thatai S; Bal V; Rath S; George A.. Oral tolerance in T cells is accompanied by induction of effector function in lymphoid organs after systemic immunization. Infect Immun，2004，72(7):3803-11. 3Abboud G; Staumont-Salle D; Kanda A; Roumier T; Deruytter N; Lavogiez C; Fleury S; Remy P; Papin JP; Capron M; Dombrowicz D.. Fc(epsilon)RI and FcgammaRIII/CD16 differentially regulate atopic dermatitis in mice. J Immunol，2009，182(10):6517-26. 4Abou Fakher FH; Rachinel N; Klimczak M; Louis J; Doyen N.. TLR9-dependent activation of dendritic cells by DNA from Leishmania major favors Th1 cell development and the resolution of lesions. J Immunol，2009，182(3):1386-96.

免疫系统相关疾病

胚胎干细胞打靶

1Martin-Orozco N; Chen Z; Poirot L; Hyatt E; Chen A; Kanagawa O; Sharpe A; Mathis D; Benoist C. Paradoxical dampening of anti-islet self-reactivity but promotion of diabetes by OX40 ligand. J Immunol，2003，171(12):6954-60. 2Chen AI; McAdam AJ; Buhlmann JE; Scott S; Lupher ML Jr; Greenfield EA; Baum PR; Fanslow WC; Calderhead DM; Freeman GJ; Sharpe AH. Ox40-ligand has a critical costimulatory role in dendritic cell:T cell interactions. Immunity，1999，11(6):689-98. 3Arestides RS; He H; Westlake RM; Chen AI; Sharpe AH; Perkins DL; Finn PW. Costimulatory molecule OX40L is critical for both Th1 and Th2 responses in allergic inflammation. Eur J Immunol，2002，32(10):2874-80.

免疫系统相关疾病

胚胎干细胞打靶

1Alexopoulou L; Holt AC; Medzhitov R; Flavell RA. 2001. Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature 413(6857):732-8. 2Hoebe K; Janssen EM; Kim SO; Alexopoulou L; Flavell RA; Han J; Beutler B. 2003. Upregulation of costimulatory molecules induced by lipopolysaccharide and double-stranded RNA occurs by Trif-dependent and Trif-independent pathways. Nat Immunol 4(12):1223-9. Aeffner F; Traylor ZP; Yu EN; Davis IC. 2011. Double-stranded RNA induces similar pulmonary dysfunction to respiratory syncytial virus in BALB/c mice. Am J Physiol Lung Cell Mol Physiol 301(1):L99-L109.

免疫系统相关疾病

胚胎干细胞打靶

1. Hogquist KA; Jameson SC; Heath WR; Howard JL; Bevan MJ; Carbone FR. 1994. T cell receptor antagonist peptides induce positive selection. Cell 76(1):17-27. 2.Chen W; Jin W; Tian H; Sicurello P; Frank M; Orenstein JM; Wahl SM. 2001. Requirement for transforming growth factor beta1 in controlling T cell apoptosis. J Exp Med 194(4):439-53. 3.Dillon S; Agrawal A; Van Dyke T; Landreth G; McCauley L; Koh A; Maliszewski C; Akira S; Pulendran B. 2004. A Toll-like receptor 2 ligand stimulates Th2 responses in vivo, via induction of extracellular signal-regulated kinase mitogen-activated protein kinase and c-Fos in dendritic cells. J Immunol 172(8):4733-43. 4.Geissmann F; Jung S; Littman DR. 2003. Blood monocytes consist of two principal subsets with distinct migratory properties. Immunity 19(1):71-82.

免疫系统相关疾病

胚胎干细胞打靶

1Hogquist KA; Jameson SC; Heath WR; Howard JL; Bevan MJ; Carbone FR. 1994. T cell receptor antagonist peptides induce positive selection. Cell 76(1):17-27. 2Chen W; Jin W; Tian H; Sicurello P; Frank M; Orenstein JM; Wahl SM. 2001. Requirement for transforming growth factor beta1 in controlling T cell apoptosis. J Exp Med 194(4):439-53. 3Dillon S; Agrawal A; Van Dyke T; Landreth G; McCauley L; Koh A; Maliszewski C; Akira S; Pulendran B. 2004. A Toll-like receptor 2 ligand stimulates Th2 responses in vivo, via induction of extracellular signal-regulated kinase mitogen-activated protein kinase and c-Fos in dendritic cells. J Immunol 172(8):4733-43.

免疫系统相关疾病

胚胎干细胞打靶

1Finotto S; Neurath MF; Glickman JN; Qin S; Lehr HA; Green FH; Ackerman K; Haley K; Galle PR; Szabo SJ; Drazen JM; De Sanctis GT; Glimcher LH.. Development of spontaneous airway changes consistent with human asthma in mice lacking T-bet. Science，2002，295(5553):336-8. 2Szabo SJ; Sullivan BM; Stemmann C; Satoskar AR; Sleckman BP; Glimcher LH.. Distinct effects of T-bet in TH1 lineage commitment and IFN-gamma production in CD4 and CD8 T cells. Science，2002，295(5553):338-42. 3Albrecht I; Niesner U; Janke M; Menning A; Loddenkemper C; Kuhl AA; Lepenies I; Lexberg MH; Westendorf K; Hradilkova K; Grun J; Hamann A; Epstein JA; Chang HD; Tokoyoda K; Radbruch A.. Persistence of effector memory Th1 cells is regulated by Hopx. Eur J Immunol，2010，40(11):2993-3006. 4Alcaide P; Jones TG; Lord GM; Glimcher LH; Hallgren J; Arinobu Y; Akashi K; Paterson AM; Gurish MA; Luscinskas FW.. Dendritic cell expression of the transcription factor T-bet regulates mast cell progenitor homing to mucosal tissue. J Exp Med，2007，204(2):431-9.

免疫系统相关疾病

胚胎干细胞打靶

1Belanger SD; St-Pierre Y. 2005. Role of selectins in the triggering, growth, and dissemination of T-lymphoma cells: implication of L-selectin in the growth of thymic lymphoma. Blood 105(12):4800-6. 2Eriksson EE. 2008. No detectable endothelial- or leukocyte-derived L-selectin ligand activity on the endothelium in inflamed cremaster muscle venules. J Leukoc Biol 84(1):93-103. 3Frenette PS; Mayadas TN; Rayburn H; Hynes RO; Wagner DD. 1996. Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell 84(4):563-74. Fujita T; Fujimoto M; Matsushita T; Shimada Y; Hasegawa M; Kuwano Y; Ogawa F; Takehara K; Sato S. 2007. Phase-Dependent Roles of E-Selectin during Chronic Contact Hypersensitivity Responses. Am J Pathol 170(5):1649-58.

传染病

克隆的人SCARB2基因插入到pXL-5载体的下游，由CMV启动子启动表达。

[1].Zhou D, Zhao Y, Kotecha A, Fry EE, Kelly JT, Wang X, Rao Z, Rowlands DJ, Ren J, Stuart DI. Unexpected mode of engagement between enterovirus 71 and its receptor SCARB2. Nat Microbiol. 2019 Mar;4(3):414-419. doi: 10.1038/s41564-018-0319-z. Epub 2018 Dec 10. PMID: 30531980. [2].Zhang X, Yang P, Wang N, Zhang J, Li J, Guo H, Yin X, Rao Z, Wang X, Zhang L. The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection. Protein Cell. 2017 Aug;8(8):590-600. doi: 10.1007/s13238-017-0405-7. Epub 2017 Apr 26. PMID: 28447294; PMCID: PMC5546930.

传染病

本项目为了建立HCV感染的小鼠模型，克隆了人的SCARB1编码基因，将其插入pUBC载体的泛素基因启动子下游。

[1].Dreux M, Dao Thi VL, Fresquet J, Guérin M, Julia Z, Verney G, Durantel D, Zoulim F, Lavillette D, Cosset FL, Bartosch B. Receptor complementation and mutagenesis reveal SR-BI as an essential HCV entry factor and functionally imply its intra- and extra-cellular domains. PLoS Pathog. 2009 Feb;5(2):e1000310. doi: 10.1371/journal.ppat.1000310. Epub 2009 Feb 20. PMID: 19229312; PMCID: PMC2636890. Gao R, Gao W, Xu G, Xu J, Ren H. Single amino acid mutation of SR-BI decreases infectivity of hepatitis C virus derived from cell culture in a cell culture model. World J Gastroenterol. 2017 Jul 28;23(28):5158-5166. doi: 10.3748/wjg.v23.i28.5158. PMID: 28811710; PMCID: PMC5537182.

免疫系统相关疾病

胚胎干细胞打靶

1Mombaerts P; Iacomini J; Johnson RS; Herrup K; Tonegawa S; Papaioannou VE. 1992. RAG-1-deficient mice have no mature B and T lymphocytes. Cell 68(5):869-77. 2Alexander CE; Kaye PM; Engwerda CR. 2001. CD95 is required for the early control of parasite burden in the liver of Leishmania donovani-infected mice. Eur J Immunol 31(4):1199-210. 3Allen CD; Ansel KM; Low C; Lesley R; Tamamura H; Fujii N; Cyster JG. 2004. Germinal center dark and light zone organization is mediated by CXCR4 and CXCR5. Nat Immunol 5(9):943-52. 4Alugupalli KR; Leong JM; Woodland RT; Muramatsu M; Honjo T; Gerstein RM. 2004. B1b lymphocytes confer T cell-independent long-lasting immunity. Immunity 21(3):379-90.

传染病

转基因小鼠  

[1].Murakami T, Carmona N, Ono A. Virion-incorporated PSGL-1 and CD43 inhibit both cell-free infection and transinfection of HIV-1 by preventing virus-cell binding. Proc Natl Acad Sci U S A. 2020 Apr 7;117(14):8055-8063. doi: 10.1073/pnas.1916055117. Epub 2020 Mar 19. PMID: 32193343; PMCID: PMC7148576. [2].Dole VS, Bergmeier W, Patten IS, Hirahashi J, Mayadas TN, Wagner DD. PSGL-1 regulates platelet P-selectin-mediated endothelial activation and shedding of P-selectin from activated platelets. Thromb Haemost. 2007 Oct;98(4):806-12. PMID: 17938805.

传染病模型

靶基因PSGL-1&SCARB2均位于pXL-5载体的CMV启动子下游，由CMV启动子启动表达。

[1].Zhou D, Zhao Y, Kotecha A, Fry EE, Kelly JT, Wang X, Rao Z, Rowlands DJ, Ren J, Stuart DI. Unexpected mode of engagement between enterovirus 71 and its receptor SCARB2. Nat Microbiol. 2019 Mar;4(3):414-419. doi: 10.1038/s41564-018-0319-z. Epub 2018 Dec 10. PMID: 30531980. [2].Zhang X, Yang P, Wang N, Zhang J, Li J, Guo H, Yin X, Rao Z, Wang X, Zhang L. The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection. Protein Cell. 2017 Aug;8(8):590-600. doi: 10.1007/s13238-017-0405-7. Epub 2017 Apr 26. PMID: 28447294; PMCID: PMC5546930. [3].Murakami T, Carmona N, Ono A. Virion-incorporated PSGL-1 and CD43 inhibit both cell-free infection and transinfection of HIV-1 by preventing virus-cell binding. Proc Natl Acad Sci U S A. 2020 Apr 7;117(14):8055-8063. doi: 10.1073/pnas.1916055117. Epub 2020 Mar 19. PMID: 32193343; PMCID: PMC7148576. [4].Dole VS, Bergmeier W, Patten IS, Hirahashi J, Mayadas TN, Wagner DD. PSGL-1 regulates platelet P-selectin-mediated endothelial activation and shedding of P-selectin from activated platelets. Thromb Haemost. 2007 Oct;98(4):806-12. PMID: 17938805.