| 标识符 | CSTR:16397.09.0H01000658 |
|---|---|
| 资源中文名称 | 脑组织特异表达MKP-1C258S突变转基因小鼠 |
| 资源英文名称 | C57BL/6J-TgN(PDGF-hMKP1C258S)-GC/ILAS |
| 疾病概述 | 神经系统疾病模型 |
| 实验动物背景信息 | C57BL/6 |
| 模型制作方法 |  |
| 模型表型数据 | MKPS是一种双重底物特异性的蛋白磷酸酶，能使MAPK上的苏氨酸/酪氨酸去磷酸化，其基因中具有编码-HCXAGXXR（S/T）G-的核苷酸序列。MKP-1是第一个克隆的丝裂原活化蛋白激酶磷酸酯酶, 识别TEY、TPY和TGY 等氨基酸残基构成的磷酸化位点，最初的研究证实它是细胞G0/G1转化的生长因子。最新的研究证实MKP-1可以抑制细胞凋亡，是一种应激蛋白，在应激反应中可以抑制ERK、JNK和P38的活性。 |
| 动物模型的评价与验证 | MKP-1C258S 突变基因表达的蛋白酶活性丢失，可竞争性的抑制野生型MKP-1的活性，PDGF启动子脑组织特异表达的hMKP-1C258S突变基因的转基因小鼠是研究MKP-1 对MAPK、JNK/SAPK、p38、ERK5/BMK1等因子调控在脑系统疾病机制的小鼠模型。 |
| 保存方式 | 冷冻 |
| 合作方式 | 不限定 |
| 相关文章 |
1 Clark AR. MAP kinase phosphatase 1: a novel mediator of biological effects of glucocorticoids[J]?? J Endocrinol, 2003,178:5-12. 2 Arooq A, Zhou MM. Structure and regulation of MAPK phosphatases[J]. Cell Signal.2004,16:769-79. 3 Hunter JJ, Tanaka N, Rockman HA, et al. Ventricular expression of a MLC-2v-ras fusion gene induces cardiac hypertrophy in transgenic mice. J Boil Chem.1995, 270: 23173-23178. 4 Xu Q, Konta T, Nakayama K, et al. Cellular defense against H2O2-induced apoptosis via MAP kinase-MKP-1 pathway. Free Radic Biol Med.,2004,36:985-93. 5 Kim GS,Kim WK, Han BH，et al. MKP-1 contributes to oxidative stress-induced apoptosis via inactivation of ERK1/2 in SH-SY5Y cells. Biochem Biophys Res Commun, 2005,338:1732-8. 6 Choi BH, Hur EM, Lee JH,et al. Protein kinase C delta-mediated proteasomal degradation of MAP kinase phosphatase-1 contributes to glutamate-induced neuronal cell death[J].J Cell Sci,2006,119:1329-40. 7 Mizuno R, Oya M, Shiomi T, et al. Inhibition of MKP-1 expression potentiates JNK related apoptosis in renal cancer cells. J Urol.2004, 172:723-7. 8 Clark AR. MAP kinase phosphatase 1: a novel mediator of biological effects of glucocorticoids[J]?? J Endocrinol, 2003,178:5-12. 9 Julie Wu and Anton M. Bennett. Essential Role for Mitogen-activated protein (MAP) kinase phosphatase-1 in stress-responsive MAP Kinase and cell survival signaling[J]. J Biol Chem.2005,280: 16461-16466. |
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